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New tools for recombinant protein production in Escherichia coli : A 5‐year update
(John Wiley & Sons Inc, 2019-07)
The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, ...
Affinity purification of recombinant proteins using a LysM domain and bacterium like particles
(Elsevier Science, 2018-08)
The lysin motif (LysM) is a ubiquitous motif across kingdoms, which in bacteria allows cell wall degrading enzymes to bind noncovalently to peptidoglycan. This property has been exploited for two decades to design mucosal ...
Biotechnological applications of occlusion bodies of Baculoviruses
(Springer, 2018-08)
The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main ...
Expression and structure-function analysis of de, a sperm cysteine rich secretory protein that mediates gamete fusion
(Society for the Study of Reproduction, 2002-04)
Rat sperm epididymal glycoprotein DE belongs to the cysteine- rich secretory protein (CRISP) family and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. To investigate the ...
Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast
(Springer, 2009-11)
Cervical cancer linked to infection with human papillomavirus (HPV) is the third cause of cancer-related death in women. As the virus cannot be propagated in culture, vaccines have been based on recombinant antigens with ...
Biotechnological applications of occlusion bodies of Baculoviruses
(Springer, 2018-08)
The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main ...
Novel intein-based self-cleaving affinity tag for recombinant protein production in Escherichia coli
(Elsevier Science, 2021-05)
We evaluated several intein-based self-cleaving affinity tags for expression and single-step affinity chromatography purification of recombinant proteins produced in Escherichia coli. We used human growth hormone (hGH) as ...
Production of Mycobacterium bovis Antigens Included in Recombinant Occlusion Bodies of Baculovirus
(Karger, 2020-07)
Bovine tuberculosis (bTB) is a disease produced by Mycobacterium bovis that affects livestock, wild animals, and humans. The classical diagnostic method to detect bTB is measuring the response induced with the intradermal ...
The fusion of Toxoplasma gondii SAG1 vaccine candidate to Leishmania infantum heat shock protein 83-kDa improves expression levels in tobacco chloroplasts
(Wiley, 2015-05)
Chloroplast transformation technology has emerged as an alternative platform offering many advantages over nuclear transformation. SAG1 is the main surface antigen of the intracellular parasite Toxoplasma gondii and a ...