info:eu-repo/semantics/article
New tools for recombinant protein production in Escherichia coli : A 5‐year update
Fecha
2019-07Registro en:
Rosano, German Leandro; Morales, Enrique Salvador; Ceccarelli, Eduardo Augusto; New tools for recombinant protein production in Escherichia coli : A 5‐year update; John Wiley & Sons Inc; Protein Science; 28; 8; 7-2019; 1412-1422
0961-8368
CONICET Digital
CONICET
Autor
Rosano, German Leandro
Morales, Enrique Salvador
Ceccarelli, Eduardo Augusto
Resumen
The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.