info:eu-repo/semantics/article
Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast
Fecha
2009-11Registro en:
Morgenfeld, Mauro Miguel; Segretin, Maria Eugenia; Wirth, Sonia Alejandra; Lentz, Ezequiel Matias; Zelada, Alicia Mercedes; et al.; Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast; Springer; Molecular Biotechnology; 43; 3; 11-2009; 243-249
1073-6085
CONICET Digital
CONICET
Autor
Morgenfeld, Mauro Miguel
Segretin, Maria Eugenia
Wirth, Sonia Alejandra
Lentz, Ezequiel Matias
Zelada, Alicia Mercedes
Mentaberry, Alejandro Nestor
Gissmann, Lutz
Bravo Almonacid, Fernando Felix
Resumen
Cervical cancer linked to infection with human papillomavirus (HPV) is the third cause of cancer-related death in women. As the virus cannot be propagated in culture, vaccines have been based on recombinant antigens with inherited high-cost production. In a search of alternative cheap production system, E7 HPV type 16 protein, an attractive candidate for anticancer vaccine development, was engineered to be expressed in tobacco chloroplast. In addition, E7 coding sequence was fused to potato virus X coat protein (CP) to compare expression level. Results show that E7CP transcript accumulation reached lower levels than non-fused E7. However, antigen expression levels were higher for fusion protein indicating that CP stabilizes E7 peptide in the chloroplast stroma. These results support viability of transplastomic plants for antigen production and the relevance of improving recombinant peptide stability for certain transgenes to enhance protein accumulation in this organelle.