Article
In vitro and in vivo models for studying Zika virus biology
Registro en:
PENA, Lindomar José et al. In vitro and in vivo models for studying Zika virus biology. The Journal of General Virology, v. 99, n. 12, p. 1529–1550, 2018.
0022-1317
10.1099/jgv.0.001153
Autor
Pena, Lindomar José
Guarines, Klarissa Miranda
Silva, Anna Jéssica Duarte
Leal, Lígia Rosa Sales
Félix, Daniele Mendes
Silva, Adalúcia
Oliveira, Sheilla Andrade de
Ayres, Constância Flávia Junqueira
Silva Júnior, Abelardo
Freitas, Antonio Carlos de
Resumen
The emergence and rapid spread of Zika virus (ZIKV) in the Americas has prompted the development of in vitro and in vivo models to understand several aspects of ZIKV biology and boost the development of vaccines and antivirals. In vitro model studies include reverse genetics systems, two-dimensional (2D) cell models, such as primary cells and cell lines, and ex vivo three-dimensional (3D) models derived from skin, brain and placenta. While these models are cost-effective and allow rigorous control of experimental variables, they do not always recapitulate in vivo scenarios. Thus, a number of in vivo models have been developed, including mosquitoes (Aedes sp. and Culex sp.), embryonated chicken eggs, immunocompetent and immunodeficient mice strains, hamsters, guinea pigs, conventional swine and non-human primates. In this review, we summarize the main research systems that have been developed in recent years and discuss their advantages, limitations and main applications. 2050-01-01
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