Article
Antioxidant Activity of Stryphnodendron rotundifolium Mart. Stem Bark Fraction in an Iron Overload Model
Registro en:
SALAZAR, Gerson Javier Torres et al. Antioxidant Activity of Stryphnodendron rotundifolium Mart. Stem Bark Fraction in an Iron Overload Model. Foods, 2021.
2304-8158
10.3390/foods10112683
Autor
Salazar, Gerson Javier Torres
Dias, Francisco Junio
Ribeiro, Paulo Riceli Vasconcelos
Brito, Edy Sousa de
Canuto, Kirley Marques
Coutinho, Henrique Douglas Melo
Ribeiro Filho, Jaime
Gallo, Monica
Montesano, Domenico
Naviglio, Daniele
Zengin, Gokhan
Costa, José Galberto Martins da
Resumen
Stryphnodendron rotundifolium Mart., popularly known as “barbatimão”, is a plant species
traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases.
Considering the well-described antioxidant properties of this species, this study investigated the
protective effects of its keto-aqueous extract using an in vitro model of iron overload. Phenolic compounds
were quantified and identified by Ultra-Performance Liquid Chromatography coupled with
quadrupole Time-Of-Flight Electrospray Ionization Mass Spectrometry (UPLC–ESI-qTOF-MS/MS)
in positive and negative ions mode analysis. Antioxidant activity was analyzed following the
iron-chelating–reducing capacity and deoxyribose degradation (2-DR) protection methods. The
analysis identified condensed tannins (54.8 mg catechin/g dry fraction (DF), polyphenols (25 mg
gallic acid/g DF), and hydrolyzable tannins (28.8 mg tannic acid/g DF). Among the constituents,
prodelphinidin, procyanidin, and prorobinetinidine were isolated and identified. The extract significantly
protected 2-DR degradation induced by Fe2+ (72% protection) or OH (43% protection).
The ortho-phenanthroline test revealed Fe2+-chelating and Fe3+-reducing activities of 93% and 84%,
respectively. A preliminary toxicological analysis using Artemia salina revealed mortality below 10%,
at a concentration of 0.25 mg/mL, indicating low toxicity under the present experimental conditions.
In conclusion, the findings of the present study indicate that Stryphnodendron rotundifolium is a source
of antioxidant compounds with the potential to be used in drug development in the context of iron
overload disorders, which remains to be further investigated in vivo.