Antioxidant Activity of Stryphnodendron rotundifolium Mart. Stem Bark Fraction in an Iron Overload Model

dc.creatorSalazar, Gerson Javier Torres
dc.creatorDias, Francisco Junio
dc.creatorRibeiro, Paulo Riceli Vasconcelos
dc.creatorBrito, Edy Sousa de
dc.creatorCanuto, Kirley Marques
dc.creatorCoutinho, Henrique Douglas Melo
dc.creatorRibeiro Filho, Jaime
dc.creatorGallo, Monica
dc.creatorMontesano, Domenico
dc.creatorNaviglio, Daniele
dc.creatorZengin, Gokhan
dc.creatorCosta, José Galberto Martins da
dc.date2021-12-09T18:56:58Z
dc.date2021-12-09T18:56:58Z
dc.date2021
dc.date.accessioned2023-09-26T23:02:43Z
dc.date.available2023-09-26T23:02:43Z
dc.identifierSALAZAR, Gerson Javier Torres et al. Antioxidant Activity of Stryphnodendron rotundifolium Mart. Stem Bark Fraction in an Iron Overload Model. Foods, 2021.
dc.identifier2304-8158
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50261
dc.identifier10.3390/foods10112683
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886198
dc.descriptionStryphnodendron rotundifolium Mart., popularly known as “barbatimão”, is a plant species traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases. Considering the well-described antioxidant properties of this species, this study investigated the protective effects of its keto-aqueous extract using an in vitro model of iron overload. Phenolic compounds were quantified and identified by Ultra-Performance Liquid Chromatography coupled with quadrupole Time-Of-Flight Electrospray Ionization Mass Spectrometry (UPLC–ESI-qTOF-MS/MS) in positive and negative ions mode analysis. Antioxidant activity was analyzed following the iron-chelating–reducing capacity and deoxyribose degradation (2-DR) protection methods. The analysis identified condensed tannins (54.8 mg catechin/g dry fraction (DF), polyphenols (25 mg gallic acid/g DF), and hydrolyzable tannins (28.8 mg tannic acid/g DF). Among the constituents, prodelphinidin, procyanidin, and prorobinetinidine were isolated and identified. The extract significantly protected 2-DR degradation induced by Fe2+ (72% protection) or OH (43% protection). The ortho-phenanthroline test revealed Fe2+-chelating and Fe3+-reducing activities of 93% and 84%, respectively. A preliminary toxicological analysis using Artemia salina revealed mortality below 10%, at a concentration of 0.25 mg/mL, indicating low toxicity under the present experimental conditions. In conclusion, the findings of the present study indicate that Stryphnodendron rotundifolium is a source of antioxidant compounds with the potential to be used in drug development in the context of iron overload disorders, which remains to be further investigated in vivo.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectAntioxidantes
dc.subjectRadicais livres
dc.subjectSobrecarga de ferro
dc.subjectStryphnodendron
dc.subjectTaninos
dc.subjectAntioxidant activity
dc.subjectFree radicals
dc.subjectIron overload
dc.subjectStryphnodendron rotundifolium
dc.subjectTannins
dc.titleAntioxidant Activity of Stryphnodendron rotundifolium Mart. Stem Bark Fraction in an Iron Overload Model
dc.typeArticle


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