Development and validation of a High Performance Liquid Chromatography-Diode Array Detection method for the determination of aging markers in tequila

Abstract

A solid-phase extraction procedure followed by analysis by high performance liquid chromatography (HPLC) with UV-vis photodiode array detection (DAD) is proposed to simultaneously determine 11 aging markers in tequila. The method showed good intraday (n = 5) and interday (n = 3) precision, RSD < 1.6% in both cases, for each of the identified compounds. The calibration curves were linear at the tested ranges (R2 > 0.999). Good recoveries (84.2-108.5%) were obtained for 10 of the 11 compounds studied; and the LOD and LOQ ranged from 0.62 to 4.09 ?g/mL and 1.9-12.4 ?g/mL, respectively. The proposed methodology was applied to a set of 15 authentic tequila samples grouped by aging state (blanco, reposado and a�ejo). An ANOVA analysis combined with discriminant analysis with stepwise backward variable selection was used to differentiate between the various aging groups based on their oak related compounds content. � 2008 Elsevier B.V. All rights reserved.