Artículo
Enzymatic preparation of structured triacylglycerides containing γ-linolenic acid
Autor
LOPEZ MARTINEZ, JOSE EDUARDO; 627208
ROMERO ROMERO, RUBI; 121454
NATIVIDAD RANGEL, REYNA; 87755
Torres Olivares, Carlos;x1349052
Garcia Galindo, Hugo Sergio; 318
BAEZA JIMENEZ, RAMIRO; 209622
LOPEZ MARTINEZ, JOSE EDUARDO
ROMERO ROMERO, RUBI
NATIVIDAD RANGEL, REYNA
Torres Olivares, Carlos
Garcia Galindo, Hugo Sergio
BAEZA JIMENEZ, RAMIRO
Institución
Resumen
with the addition of molecular sieves (MS) at different addition times (3, 9, 12 and 15 h) and loads (2.5, 5, 10 and 15%, based on the total weight of substrates), the enrichment of GLA from evening primrose oil (EPO) and 1- butanol (BtOH) was improved via Candida rugosa lipase-catalysed esterification reactions. Secondly, the GLAenriched fraction was separated by thin-layer chromatography (TLC) to be further set to react during the third step in the presence of glycerol and Candida antarctica fraction B (CALB), under different enzyme loadings (5, 10, 15 and 20%, based on the total weight of substrates), temperatures (30, 40, 50 and 60 ◦C) and substrates molar ratios (1:1, 2:1, 3:1 and 4:1, GLA:glycerol). 60% of STAG containing 49 wt% of GLA were produced by using 15% of CALB at 60 ◦C and a 3:1 molar ratio. Conacyt: Beca no. 328716 UAEMex: Proyecto 3866/2015/PIC