Artículo de revista
One-electron reduction of 6-hydroxydopamine quinone is essential in 6-hydroxydopamine neurotoxicity
Fecha
2013Registro en:
Neurotoxicity Research, Volumen 24, Issue 1, 2018, Pages 94-101
10298428
14763524
10.1007/s12640-013-9382-7
Autor
Villa, Monica
Muñoz, Patricia
Ahumada-Castro, Ulises
Paris, Irmgard
Jiménez, Ana
Martínez, Isabel
Sevilla, Francisca
Segura Aguilar, Juan
Institución
Resumen
6-Hydroxydamine has widely been used as neurotoxin in preclinical studies related on the neurodegenerative process of dopaminergic neurons in Parkinson's disease based on its ability to be neurotoxic as a consequence of free radical formation during its auto-oxidation to topaminequinone. We report that 50-μM 6-hydroxydopamine is not neurotoxic in RCSN-3 cells derived from substantia nigra incubated during 24 h contrasting with a significant sixfold increase in cell death (16 ± 2 %; P < 0.001) was observed in RCSN-3NQ7 cells expressing a siRNA against DT-diaphorase that silence the enzyme expression. To observe a significant cell death in RCSN-3 cells induced by 6-hydroxydopamine (24 ± 1 %; P < 0.01), we have to increase the concentration to 250 μm while a 45 ± 2 % cell death (P < 0.001) was observed at this concentration in RCSN-3NQ7 cells. The cell death induced by 6-hydroxydopamine in RCSN-3NQ7 cells was accompanied with a (i) significant increase in oxygen consumption (P < 0.01), (i