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Quantitative PCR and unconventional serological methods to evaluate clomipramine treatment effectiveness in experimental Trypanosoma cruzi infection
(Academic Press Inc Elsevier Science, 2016-10)
Clomipramine (CLO), a tricyclic antidepressant drug, has been used for the treatment of mice infected with Trypanosoma cruzi. In this work we evaluated the effectiveness of CLO treatment upon T. cruzi-infected mice in the ...
Identification and evaluation of antioxidant and reference genes for quantitative real-time PCR in blood of Caiman latirostris
(Elsevier Science, 2021-02)
The quantitative real-time polymerase chain reaction (qPCR) has been one of the most promising approaches to perform rapid and accurate quantification of DNA in various biological systems. The aim of this study was to ...
Impact of extracellular nucleic acids from lactic acid bacteria on qPCR and RT-qPCR results in dairy matrices: Implications for defining molecular markers of cell integrity
(Elsevier Science, 2017-07)
In this study, we evaluated whether quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) results could be affected by extracellular (free) DNA or RNA, respectively, obtained either from isolated bacteria or ...
Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium
(Springer, 2021-04-02)
Despite the intensive use of glyphosate (GP) and its ubiquitous presence in the environment, studies addressing the presence of microbial genes involved in glyphosate degradation in natural conditions are scarce. Based on ...
Bacterial genomic dna extraction: a comparison of commercial and in house methodsExtração de dna genômico bacteriano: uma comparação de métodos comerciais e in house
(Universidade Federal de Santa Maria, 2020)
Characterization of specific cDNA background synthesis introduced by reverse transcription in RT-PCR assays
(Elsevier France-editions Scientifiques Medicales Elsevier, 2010-12)
To block expression of NMDA receptor NR1 subunit, we injected into rat hippocampus a Herpes Simplex Virus type 1 derived vector bearing a sequence for NR1 antisense. RT-PCR assays with RNA from hippocampus of animals ...
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters
(Wiley, 2021-01)
Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in ...
Bacteroidales markers for microbial source tracking in Southeast Asia
(Pergamon-Elsevier Science Ltd, 2017-04)
The island city country of Singapore served as a model to validate the use of host-associated Bacteroidales 16S rRNA gene marker assays for identifying sources of fecal pollution in the urban tropical environment of Southeast ...
Selection and optimization of reference genes for RT-qPCR normalization: a case study in Solanum lycopersicum exposed to UV-B
(Elsevier France-Editions Scientifiques Medicales Elsevier, 2021-03-22)
Quantitative RT- PCR is one of the most common methods to study gene expression in response to stress. Therefore, it is crucial to have suitable reference genes (RGs) for result normalization. Although several reports ...
Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata
(Institute of Malacology, 2018-03)
The South American freshwater gastropod Pomacea canaliculata is a highly invasive species. In introduced areas, it is a serious crop pest, responsible for great economic loss and ecological damage. It is also a vector of ...