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Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
(BioMedCentral, 2018-07-27)
OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this ...
Development of a suitable manufacturing process for production of a bioactive recombinant equine chorionic gonadotropin (reCG) in CHO-K1 cells
(Elsevier Science Inc., 2021-09)
Equine chorionic gonadotropin (eCG) is a heterodimeric glycoprotein hormone produced by pregnant mares that has been used to improve reproductive performance in different domestic species. Several strategies to produce the ...
Diseño y producción de vectores lentivirales para modular la expresión de genes involucrados en la inmunosupresión
(Sociedad Argentina de Virología, 2019)
La proteína STUB1 fue recientemente caracterizada como una enzima de tipo E3 ligasa, capaz de promover la degradación del factor de transcripción FOXP3 de manera específica. Es ampliamente conocido que FOXP3 tiene un rol ...
Silencing brain catalase expression reduces ethanol intake in developmentally-lead-exposed rats
(Elsevier Science, 2019-06)
Lead (Pb) is a developmental neurotoxicant. We have demonstrated that perinatally Pb-exposed rats consumemore ethanol than their control counterparts, a response that seems to be mediated by catalase (CAT) andcentrally-formed ...
Generation of a human induced pluripotent stem cell line from a familial Alzheimer's disease PSEN1 T119I patient
(Elsevier, 2021-05)
Human induced pluripotent stem cells (hiPSC) line FLENIi001-A was reprogrammed from dermal fibroblasts using the lentiviral-hSTEMCCA-loxP vector. Fibroblasts were obtained from a skin biopsy of a 72-year-old Caucasian male ...
Silencing of cardiac mitochondrial NHE1 prevents mitochondrial permeability transition pore opening
(American Physiological Society, 2011-04)
Inhibition of Na +/H + exchanger 1 (NHE1) reduces cardiac ischemia-reperfusion (I/R) injury and also cardiac hypertrophy and failure. Although the mechanisms underlying these NHE1-mediated effects suggest delay of mitochondrial ...
Lentivector Transduction Improves Outcomes Over Transplantation of Human HSCs Alone in NOD/SCID/Fabry Mice
(Nature Publishing Group, 2012-07)
Fabry disease is a lysosomal storage disorder caused by a deficiency of a-galactosidase A (a-gal A) activity that results in progressive globotriaosylceramide (Gb(3)) deposition. We created a fully congenic nonobese diabetic ...
Gene Therapy Approaches using Reproducible and Fully Penetrant Lentivirus-Mediated Endogenous Glioma Models
(Springer New York, 2015)
Animal models have proven invaluable for progress toward greater understanding of the etiology, pathogenesis, and genetics of a wide range of human diseases. The development of relevant brain tumor animal models is a ...
A Novel Splice Variant of Human TGF-β Type II Receptor Encodes a Soluble Protein and Its Fc-Tagged Version Prevents Liver Fibrosis in vivo
(Frontiers Media, 2021-09-10)
We describe, for the first time, a new splice variant of the human TGF-β type II receptor (TβRII). The new transcript lacks 149 nucleotides, resulting in a frameshift and the emergence of an early stop codon, rendering a ...
Myocardial mineralocorticoid receptor activation by stretching and its functional consequences
(Lippincott Williams, 2014-01)
Myocardial stretch triggers an angiotensin II-dependent autocrine/paracrine loop of intracellular signals, leading to reactive oxygen species-mediated activation of redox-sensitive kinases. Based on pharmacological strategies, ...