info:eu-repo/semantics/article
Lentivector Transduction Improves Outcomes Over Transplantation of Human HSCs Alone in NOD/SCID/Fabry Mice
Fecha
2012-07Registro en:
Pacienza, Natalia Alejandra; Yoshimitsu, Makoto; Mizue, Nobuo; Au, Bryan C. Y.; Wang, James C. M.; et al.; Lentivector Transduction Improves Outcomes Over Transplantation of Human HSCs Alone in NOD/SCID/Fabry Mice; Nature Publishing Group; Molecular Therapy (print); 20; 7; 7-2012; 1454-1461
1525-0016
CONICET Digital
CONICET
Autor
Pacienza, Natalia Alejandra
Yoshimitsu, Makoto
Mizue, Nobuo
Au, Bryan C. Y.
Wang, James C. M.
Fan, Xin
Takenaka, Toshihiro
Medin, Jeffrey A
Resumen
Fabry disease is a lysosomal storage disorder caused by a deficiency of a-galactosidase A (a-gal A) activity that results in progressive globotriaosylceramide (Gb(3)) deposition. We created a fully congenic nonobese diabetic (NOD)/severe combined immunodeficiency (SCID)/Fabry murine line to facilitate the in vivo assessment of human cell-directed therapies for Fabry disease. This pure line was generated after 11 generations of backcrosses and was found, as expected, to have a reduced immune compartment and background a-gal A activity. Next, we transplanted normal human CD34(+) cells transduced with a control (lentiviral vector-enhanced green fluorescent protein (LV-eGFP)) or a therapeutic bicistronic LV (LV-a-gal A/internal ribosome entry site (IRES)/hCD25). While both experimental groups showed similar engraftment levels, only the therapeutic group displayed a significant increase in plasma a-gal A activity. Gb(3) quantification at 12 weeks revealed metabolic correction in the spleen, lung, and liver for both groups. Importantly, only in the therapeutically-transduced cohort was a significant Gb(3) reduction found in the heart and kidney, key target organs for the amelioration of Fabry disease in humans.