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Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila
(Elsevier Science Sa, 2018-10)
5-fluorouracil-2’-deoxyriboside (FUdR), an antimetabolite known as floxuridine, is a halogenated nucleoside extensively used in the clinical treatment of colon carcinoma and hepatic metastases. This drug presents low ...
One-Step Immobilization and Stabilization of a Recombinant Enterococcus faecium DBFIQ E36 l-Arabinose Isomerase for d-Tagatose Synthesis
(Humana Press, 2019-04)
A recombinant l-arabinose isomerase from Enterococcus faecium DBFIQ E36 was immobilized onto multifunctional epoxide supports by chemical adsorption and onto a chelate-activated support via polyhistidine-tag, located on ...
Studies on the effect of temperature on the activity and stability of cyanobacterial ADP-glucose pyrophosphorylase
(Elsevier Science Inc, 2000-12)
The effect of temperature on the activity and stability of ADPglucose pyrophosphorylase from Anabaena PCC 7120 was studied. Experimental optima temperatures were found around 37-40°C or 42-45°C, depending on the absence ...
Stability of Araujiain, a novel plant protease, in different organic systemsEstabilidad de Araujiaína, una nueva fitoproteasa, en diferentes sistemas orgánicos
(Colegio de Farmacéuticos de la Provincia de Buenos Aires, 2005-12)
The effect of different organic solvents on the stability of araujiain(the crude enzyme preparation obtained from the latex of fruits of Araujia hortorum Fourn., Asclepiadaceae) in mixtures of buffer and different ...
Wired Enzymes in mesoporous materials: A benchmark for fabricating biofuel cells
(Elsevier Science Sa, 2015)
Determination of the β-glucosidase activity in different soils by pre capillary enzyme assay using capillary electrophoresis with laser-induced fluorescence detection
(Springer/Plenum Publishers, 2010-03)
Enzyme activities can provide indication for quantitative changes in soil organic matter (SOM). It is known that the activities of most enzymes increase as native SOM content reflecting larger microbial communities and ...
Recent advances in β-galactosidase and fructosyltransferase immobilization technology
(Taylor & Francis, 2020-06)
The highly demanding conditions of industrial processes may lower the stability and affect the activity of enzymes used as biocatalysts. Enzyme immobilization emerged as an approach to promote stabilization and easy removal ...
Green production of cladribine by using immobilized 2′-deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
(MDPI AG, 2021-04)
Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a ...
Processing of pre-cooked frozen Brussels sprouts: Heat transfer modelling as related to enzyme inactivation and quality stability
(Institution of Chemical Engineers, 2019-11)
The finite element numerical method was applied to simulate heat transfer processes (heating, cooling and freezing) in irregular domains during the production of precooked frozen Brussels sprouts; the effect of temperature ...
Opposing effects of Na+ and K+ on the thermal stability of Na+,K+-ATPase
(American Chemical Society, 2012-03)
Folding and structural stability are key factors for the proper biological function of proteins. Na+,K+-ATPase is an integral membrane protein involved in the active transport of Na+ and K+ across the plasma membrane. In ...