info:eu-repo/semantics/article
Green production of cladribine by using immobilized 2′-deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
Fecha
2021-04Registro en:
Rivero, Cintia Wanda; Garcia, Natalia Soledad; Fernández Lucas, Jesús; Betancor, Lorena; Romanelli, Gustavo Pablo; et al.; Green production of cladribine by using immobilized 2′-deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology; MDPI AG; Biomolecules; 11; 5; 4-2021; 657-660
2218-273X
CONICET Digital
CONICET
Autor
Rivero, Cintia Wanda
Garcia, Natalia Soledad
Fernández Lucas, Jesús
Betancor, Lorena
Romanelli, Gustavo Pablo
Trelles, Jorge Abel
Resumen
Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60◦ C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale.