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Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells
(BioMed Central Ltd., 2019)
Background: The production of recombinant proteins in mammalian cell lines is one of the most important areas in biopharmaceutical industry. Viral transcriptional promoters are widely used to express recombinant proteins ...
Human interferon B1 ser17: Coding DNA synthesis, expression, purification and characterization of bioactive recombinant protein
(2010-09-01)
A protocol to produce large amounts of bioactive homogeneous human interferon β1 expressed in Escherichia coli was developed. Human interferon β1 ser17 gene was constructed, cloned and subcloned, and the recombinant protein ...
Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds
(2018-07-01)
BACKGROUND: Green fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) ...
SK-HEP cells and lentiviral vector for production of human recombinant factor VIII
(SPRINGERDORDRECHT, 2012)
Hemophilia A is caused by a deficiency in coagulation factor VIII. Recombinant factor VIII can be used as an alternative although it is unavailable for most patients. Here, we describe the production of a human recombinant ...
NUCEL (Cell and Molecular Therapy Center): A multidisciplinary center for translational research in Brazil
(HUMANA PRESS INC, 2008)
Social and economical development is closely associated with technological innovation and a well-developed biotechnological industry. In the last few years, Brazil`s scientific production has been steadily increasing; ...
RECOMBINATION AS A POSSIBLE MAJOR CAUSE OF GENETIC-HETEROGENEITY IN ANTICARSIA-GEMMATALIS NUCLEAR POLYHEDROSIS-VIRUS WILD POPULATIONS
(Elsevier Science BvAmsterdamHolanda, 1992)
Recombinant human zona pellucida protein C produced in Chinese hamster ovary cells binds to human spermatozoa and inhibits sperm-zona pellucida interaction
(Elsevier Science Inc, 2008-09)
Recombinant human zona pellucida protein C expressed in Chinese hamster ovary cells associates to the acrosomal region of human spermatozoa and inhibits sperm-zona pellucida interaction in the hemizona assay. Recombinant ...
Immune response of broiler chickens immunized orally with the recombinant proteins flagellin and the subunit B of cholera toxin associated with Lactobacillus spp.
(Oxford University Press, 2014-01-01)
This study investigated the immune response of broiler chickens with oral treatment of a Lactobacillus spp. pool (PL) associated with microencapsulated recombinant proteins flagellin (FliC) and the subunit B of cholera ...
Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein
(BioMed Central, 2010-10)
Background: Multiple HIV-1 intersubtype recombinants have been identified in human populations. Previous studies from our lab group have shown that the epidemic in Argentina is characterized by the high prevalence of a ...
A critical view to explanations for the maximal value of 50 % of genetic recombination
(Sociedad de Biología de Chile, 2001)