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Purification and characterization of a novel thermostable papain inhibitor from Moringa oleifera with antimicrobial and anticoagulant properties
(Multidisciplinary Digital Publishing Institute, 2021-04)
Plant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this report, we present the purification ...
Plant serine protease inhibitors: Biotechnology application in agriculture and molecular farming
(Molecular Diversity Preservation International, 2019-03)
The serine protease inhibitors (SPIs) are widely distributed in living organisms like bacteria, fungi, plants, and humans. The main function of SPIs as protease enzymes is to regulate the proteolytic activity. In plants, ...
GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds. A novel natural drug with potential application in biomedicine
(Elsevier Science, 2020-01)
A novel thermostable trypsin inhibitor was obtained from Geoffroea decorticans seeds. G. decorticans trypsin inhibitor(GdTI) is a protein with molecular mass of 6743.7 Da, with a potent inhibitory activity (Ki of 2.1 nM) ...
PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans
(Wiley, 2014-04)
Potato leaves infected with Phytophthora infestans produced a serine protease inhibitor (PLPKI) with specificity for microbial proteases. Sequencing of the first twenty residues at the NH2‐terminus of the mature PLPKI ...
Purification and characterization of a novel trypsin inhibitor from Solanum tuberosum subsp. andigenum var. overa: Study of the expression levels and preliminary evaluation of its antimicrobial activity
(Elsevier Science, 2020-09-01)
Protease inhibitors (PIs) have been traditionally recognized by their potential biomedical application in events with exacerbation of endogenous proteases activity. Plant PIs have gained interest as naturally occurring ...
Non-digestible proteins and protease inhibitors: Implications for defense of the colored eggs of the freshwater apple snail pomacea canaliculata
(National Research Council Canada-NRC Research Press, 2019-02-01)
Apple snails (Pomacea Perry, 1810) are successful invaders that cause ecological perturbations, economic losses, and medical issues. A peculiar trait of this snail is a high biological potential, related to the absence of ...
Biochemical and MALDI-TOF mass spectrometric characterization of a novel native and recombinant cystine knot miniprotein from Solanum tuberosum subsp. andigenum cv. Churqueña
(Molecular Diversity Preservation International, 2018-03)
Cystine-knot miniproteins (CKMPs) are an intriguing group of cysteine-rich molecules that combine the characteristics of proteins and peptides. Typically, CKMPs are fewer than 50 residues in length and share a characteristic ...
Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors
(American Phytopathological Society, 2017-04)
Significant efforts are being made to minimize aflatoxin contamination in peanut seeds and one possible strategy is to understand and exploit the mechanisms of plant defense against fungal infection. In this study we have ...
Chloroplast protein degradation in senescing leaves: Proteases and lytic compartments
(Frontiers Media, 2019-06-19)
Leaf senescence is characterized by massive degradation of chloroplast proteins, yet the protease(s) involved is(are) not completely known. Increased expression and/or activities of serine, cysteine, aspartic, and ...
Hydrophobic proteins secreted into the apoplast may contribute to resistance against Phytophthora infestans in potato
(Elsevier France-editions Scientifiques Medicales Elsevier, 2012-07)
During plantepathogen interaction, oomycetes secrete effectors into the plant apoplast where they interact with host resistance proteins, which are accumulated after wounding or infection. Previous studies showed that the ...