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Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
(Sociedade Brasileira de Microbiologia, 2014-09-01)
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in ...
Genetic identification of lamniform and carcharhiniform sharks using multiplex-PCR
(Springer, 2010-12-01)
Nowadays, because of the constant increase in the capture and trade of sharks all over the world and reports of several species already showing important signs of over-exploitation, the establishment of registration ...
A multiplex PCR approach for the molecular identification and conservation of the Critically endangered daggernose shark
(2017-01-01)
The daggernose shark Isogomphodon oxyrhynchus is an inshore tropical species endemic to the coastal waters off northeastern South America with limited distribution and restrictive biological traits. According to the IUCN, ...
Simultaneous detection of bee viruses by multiplex PCR
(Elsevier Science, 2013-08)
Honey bee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. Itis known thatthe consequence ofthe complex interactions between environmental and beekeeping parameters added ...
A multiplex nested PCR for the detection and identification of Candida species in blood samples of critically ill paediatric patients
(BioMed Central, 2014)
Abstract
Background
Nosocomial candidaemia is associated with high mortality rates in critically ill paediatric patients; thus, the early detection and identification of ...
Development of a multiplex real time PCR to differentiate Sarcocystis spp. affecting cattle
(Elsevier, 2013-10-18)
Cattle are intermediate hosts of Sarcocystis cruzi, Sarcocystis hirsuta and Sarcocystis hominis which use canids, felids or primates as definitive hosts (DH), respectively, and in addition of Sarcocystis sinensis from which ...
Detection of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in Brazilian mastitic milk goats by multiplex-PCR
(2018-07-01)
This study evalueted the prevalence of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in milk samples from 257 goats (513 half-udders) and ten bulk tanks, from ten dairy goat farms of São Paulo State, ...
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates
(Sociedade Brasileira de Microbiologia, 2011-03-01)
Identification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability of the epidemiological and probiotics studies. Therefore this study aimed to determine ...
Multiplex PCR followed by restriction length polymorphism for the subtyping of bovine herpesvirus 5 isolates
(Biomed Central, 2013-06)
Abstract Background: Several types and subtypes of bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, making type/subtype differentiation essential to ...