Artículos de revistas
Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
Fecha
2014-09-01Registro en:
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 45, n. 3, p. 841-843, 2014.
1517-8382
10.1590/S1517-83822014000300012
S1517-83822014000300012
S1517-83822014000300012.pdf
2114570774349859
Autor
Universidade Estadual de Maringá (UEM)
Universidade Estadual de Maringá (UEM)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Institución
Resumen
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.