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Cytosine methylation of sperm DNA in horse semen after cryopreservation
(2016-09-15)
Semen processing may contribute to epigenetic changes in spermatozoa. We have therefore addressed changes in sperm DNA cytosine methylation induced by cryopreservation of stallion semen. The relative amount of 5-methylcytosine ...
A survey of DNA methylation across social insect species, life stages, and castes reveals abundant and caste-associated methylation in a primitively social wasp
(2013-08-01)
DNA methylation plays an important role in the epigenetic control of developmental and behavioral plasticity, with connections to the generation of striking phenotypic differences between castes (larger, reproductive queens ...
DNA methylation in sugarcane somaclonal variants assessed through methylation-sensitive amplified polymorphism
(Funpec-editora, 2017-05-08)
Micropropagation is an important tool for large-scale multiplication of plant superior genotypes. However, somaclonal variation is one of the drawbacks of this process. Changes in DNA methylation have been widely reported ...
In vitro methylation of the elongation factor EF-Tu from Escherichia coli
(1985)
The in vitro methylation of the elongation factor EF-Tu from Escherichia coli was investigated. The methylation of newly synthesized EF-Tu was obtained using lambda rifd 18 DNA as template and S-adenosyl [methyl-3H]methionine ...
Search for methylation-sensitive amplification polymorphisms in mutant figs
(2013-07-08)
Fig (Ficus carica) breeding programs that use conventional approaches to develop new cultivars are rare, owing to limited genetic variability and the difficulty in obtaining plants via gamete fusion. Cytosine methylation ...
Developmental genome-wide DNA methylation asymmetry between mouse placenta and embryo
(2020-08-02)
In early embryos, DNA methylation is remodelled to initiate the developmental program but for mostly unknown reasons, methylation marks are acquired unequally between embryonic and placental cells. To better understand ...
A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements
(Oxford University Press, 2004-02-01)
We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed nucleotide elements ...
Relationship between DNA methylation and cell proliferation in Trypanosoma cruzi
(1991)
5-Azacytidine treatment of T. cruzi epimastigotes in culture induces active cell proliferation. This effect was detected as an increase in the cell number and [3H-methyl]thymidine incorporation into DNA. 5-Azacytidine does ...
Genome-wide screening of DNA methylation in bovine blastocysts with different kinetics of development
(2018-01-08)
Background: The timing of the first cell divisions may predict the developmental potential of an embryo, including its ability to establish pregnancy. Besides differences related to metabolism, stress, and survival, embryos ...