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Prospective inference of bioprocess cell viability through chemometric modeling of fluorescence multiway data
(American Institute of Chemical Engineers, 2021-07)
In this investigation, the fermentation step of a standard mammalian cell-based industrial bioprocess for the production of a therapeutic protein was studied, with particular emphasis on the evolution of cell viability. ...
Tetrazolium salts and formazan products in Cell Biology: Viability assessment, fluorescence imaging, and labeling perspectives
(Elsevier Gmbh, Urban & Fischer Verlag, 2018-04)
For many years various tetrazolium salts and their formazan products have been employed in histochemistry and for assessing cell viability. For the latter application, the most widely used are 3-(4,5-dimethyl-2-thiazolyl ...
Cell Viability and Functionality of Probiotic Bacteria in Dairy Products
(Frontiers, 2011-05)
Probiotic bacteria, according to the definition adopted by the World Health Organization in 2002, are live microorganisms, which when administered in adequate amounts confer a health benefit to the host. Recent studies ...
Real-time single-cell imaging reveals accelerating lipid peroxyl radical formation in Escherichia coli triggered by a fluoroquinolone antibiotic
(American Chemical Society, 2020-07)
Formation of reactive oxygen species (ROS) induced by bactericidal antibiotics has been associated with a common, non-specific, mechanism of cellular death. Herein we report real-time single cell fluorescence studies on ...
Evaluation of viable cells in Pseudomonas aeruginosa biofilms by colony count and live/dead staining
(Bio-protocol, 2020-09)
Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points ...
Particulate matter cytotoxicity in cultured SH-SY5Y cells is modulated by simvastatin: Toxicological assessment for oxidative damage
(Elsevier Science, 2016-01)
Epidemiological studies have shown a positive correlation between environmental particulate matter and adverse health effects. In particular, residual oil fly ash (ROFA) induces inflammation and reactive oxygen species ...
Differential effect of heterocyclic D-ribofuranoside derivatives on human prostate cancer cell viability and cell cycle progression
(Elsevier Masson, 2014-08)
New D-ribofuranoside derivatives containing two five membered heterocycles, isoxazole and triazole or two triazole rings, were synthesized. The final products as well as the synthetic precursors were physically and ...
Evaluation of PMA-qPCR methodology to detect and quantify viable Shiga toxin-producing Escherichia coli in beef burgers
(Wiley Blackwell Publishing, Inc, 2021-02)
Propidium monoazide (PMA) is a selective nucleic acid intercalating dye that can be combined with real-time PCR (qPCR) in order to evaluate cell viability in food samples. The aim of the present work was to evaluate PMA-qPCR ...