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Molecular cloning and sequence determination of a cDNA coding for the α-subunit of a Go-type protein of Xenopus laevis oocytes
(1989)
Xenopus laevis oocytes are cells ideally suited to the study of signal transduction and of the G-proteins that are involved in this process. A X. laevis cDNA library in λgt10 has been screened with a mixture of three ...
Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing
(Co-Action Publishing, 2014)
Background and objective: The advent of next-generation sequencing has significantly facilitated characterization of the oral microbiome. Despite great efforts in streamlining the processes of sequencing and data
curation, ...
Chromosomal mapping of repetitive DNA sequences in the genus Bryconamericus (characidae) and DNA barcoding to differentiate populations
(Mary Ann Liebert, 2017-06)
The mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high ...
Molecular systematics and taxonomic status of three latitudinally widespread nototheniid (Perciformes: Notothenioidei) fishes from the Southern Ocean
(Magnolia Press, 2016)
The taxonomic status of the three nototheniids, Lepidonotothen squamifrons, L. larseni and
Gobionotothen marionensis from different localities in the Southern Ocean is re-evaluated at the
DNA level. DNA sequence divergences ...
Fast DNA sequencing
(Elsevier, 2000)
A method for fast nucleotide sequencing is described. It is based in the selection of well-known small oligomers able to be hybridized with the unknown target. The selected oligomers are afterwards ordered following a ...
Evidence of absence of Trypanosoma cruzi kinetoplast DNA methylation by restriction endonuclease analysis
(1991)
Evidence of absence of Trypanosoma cruzi kinetoplast DNA methylation by restriction endonuclease analysis. International Journal for Parasitology bd21: 863-866. Eight kinetoplast DNA samples from very different T. cruzi ...
DNA methylation in Trypanosoma cruzi
(1990)
DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. ...
Characterization of Trypanosoma cruzi MutY DNA glycosylase ortholog and its role in oxidative stress response
(Elsevier, 2017)
Trypanosoma cruzi is a protozoan parasite and the causative agent of Chagas disease. Like most living organisms, it is susceptible to oxidative stress, and must adapt to distinct environments. Hence, DNA repair is essential ...
Cloning, sequencing and expression of the cDNA of endoxylanase B from Penicillium purpurogenum
(ELSEVIER SCIENCE BV, 1997-03-18)
The cDNA, for xylanase B from Penicillium purpurogenum was cloned and sequenced. This DNA encodes a protein of 208 amino acids which is expected to yield a protein of 183 residues upon processing of the N terminus. The ...
DNA Inhibits the Catalytic Activity of the α Subunit of Protein Kinase CK2
(1995)
The recombinant α subunit of protein kinase CK2 (casein kinase 2) from Xenopus laevis is inhibited by the addition of single stranded or double stranded DNA. This inhibition is competitive with the casein substrate, having ...