Article
Performance of recombinant chimeric proteins in the serological diagnosis of Trypanosoma cruzi infection in dogs
Registro en:
LEONY, Leonardo Maia et al. Performance of recombinant chimeric proteins in the serological diagnosis of Trypanosoma cruzi infection in dogs. Plos Neglected Tropical Diseases, v. 13, n. 6, p. 1-16, 2019.
1935-2727
10.1371/journal.pntd.0007545
Autor
Leony, Leonardo Maia
Freitas, Natália Erdens Maron de
Del-Rei, Rodrigo Pimenta
Carneiro, Claudia Martins
Reis, Alexandre Barbosa
Jansen, Ana Maria
Xavier, Samanta Cristina das Chagas
Gomes, Yara M.
Silva, Edmilson D.
Reis, Mitermayer Galvão dos
Fraga, Deborah Bittencourt Mothé
Celedon, Paola Alejandra Fiorani
Zanchin, Nilson I. T.
Torres, Filipe Dantas
Santos, Fred Luciano Neves
Resumen
Gonc¸alo
Moniz Institute (Research Excellence Program -
PROEP/IGM, Grant: 400904/2013-6; Internal
Research Support Program - PIAP Jovem+, Grant:
PIAP-001/2017), and in part by the Coordination of
Superior Level Staff Improvement - Brazil (CAPES,
Grant PROEX 0720/2018). Brazilian National
Council for Scientific and Technological Development (CNPq) and Research Support
Foundation for the State of Rio de Janeiro
(FAPERJ). MGR, NITZ, CMC, ABR and AMJ are
research fellows of CNPq. This study was funded
by Oswaldo Cruz Foundation- FIOCRUZ; Vice-
Presidency of Research and Reference
Laboratories (VPPLR/FIOCRUZ) for investing in our
research; the European Union Seventh Framework
Program Grant 223034 – ChagasEpiNet. Dogs are considered sentinels in areas of Trypanosoma cruzi transmission risk to humans. ELISA is generally the method of choice for diagnosing T. cruzi exposure in dogs, but its performance substantially depends on the antigenic matrix employed. In previous studies, our group has developed four chimeric antigens (IBMP-8.1, 8.2, 8.3, and 8.4) and evaluated their potential for diagnosing T. cruzi exposure in humans. For human sera, these chimeric antigens presented superior diagnostic performances as compared to commercial tests available in Brazil, Spain, and Argentina. Therefore, in this study we have evaluated the potential of these antigenic proteins for detection of anti-T. cruzi IgG antibodies in dog sera.