Article
Structure Elucidation, Antimicrobial and Cytotoxic Activities of a Halimane Isolated from Vellozia kolbekii Alves (Velloziaceae)
Registro en:
SILVA, Carmelita G. et al. Structure Elucidation, Antimicrobial and Cytotoxic Activities of a Halimane Isolated from Vellozia kolbekii Alves (Velloziaceae). Chemistry & Biodiversity, v. 12, n. 2, p. 1891-1901, Dec. 2015.
1612-1872
10.1002/cbdv.201500071
1612-1880
Autor
Silva, Carmelita G.
Santos Júnior, Helvécio M.
Barbosa, Jussara P.
Costa, Gisela L.
Rodrigues, Felipe A. R.
Oliveira, Denilson F.
Lotufo, Letícia V. Costa
Alves, Ruy J. V.
Eleutherio, Elis C. A.
Rezende, Claudia M.
Resumen
A new halimane diterpene was isolated from Vellozia kolbekii Alves (Velloziaceae) and identified as (5R,8R,9S,13R)-halim-1,10-ene-15,16-diol (1). It showed cytotoxicity against three human cancer cell lines, SF-295 (glioblastoma), MDA-MB-435 (melanoma), and HCT-8 (colon adenocarcinoma). In the mechanism of cytotoxic action, halimane 1 interferes in two major phases of the cell cycle: in S phase, in which DNA synthesis occurs and where it is very sensitive to damage, and G2M phase which is the phase of preparation for mitosis and mitosis itself, showing apoptosis-inducing properties. Antimicrobial activity towards Gram-positive and Gram-negative bacteria was studied and, against Bacillus cereus, B. subtilis, Escherichia coli, and Pseudomonas aeruginosa, a MIC value of 0.025 μM was observed for halimane 1, which is more active than the positive control chloramphenicol.