Article
Serological and molecular evidence of hepadnavirus infection in swine
Registro en:
VIEIRA, Yasmine R. et al. Serological and molecular evidence of hepadnavirus infection in swine. Annals of Agricultural and Environmental Medicine, v. 22, n.1, p.11–16, 2015.
1232-1966
10.5604/12321966.1141361
1898-2263
Autor
Vieira, Yasmine R.
Silva, Marcelle F. M.
Santos, Débora R. L.
Vieira, Antônio A.
Zanella, Janice R. Ciacci
Barquero, Gonzalo
Lago, Bárbara V. do
Gomes, Selma A.
Pinto, Marcelo A.
Paula, Vanessa S. de
Resumen
Abstract
Introduction and objective. Recently, investigations in a swine herd identified evidence of the existence of a novel member
of the Hepadnavirus family endemic in swine. The aim of this study was to investigate the serological and molecular markers
of Hepadnavirus circulation in Brazilian domestic swine and wild boar herds, and to evaluate the identity with HBV and
other Hepadnaviruses reported previously.
Materials and methods. For the study, 376 swine were screened for hepatitis B virus serological markers. Analyses were
performed in serum samples using commercial enzyme-linked immunosorbent assay (ELISA) kits (DiaSorin®) for anti-HBc,
HBsAg and anti-HBs. Reactive and undetermined swine serum samples were selected to perform DNA viral extraction
(QIAamp DNA Mini Kit, Qiagen®), partial genome amplification and genome sequencing.
Results. From 376 swine samples analysed, 28 (7.45%) were reactive to anti-HBc, 3 (0.80%) to HBsAg and 6 (1.6%) to anti-HBs.
Besides, more 17 (4.52%) swine samples analyzed were classified in the grey zone of the EIA test to anti-HBc and 2 (0.53%) to
HBsAg. From 49 samples molecularly analyzed after serological trial, 4 samples showed a positive result for the qualitative
PCR for Hepadnavirus. Phylogenetic reconstruction using partial genome sequencing (360 bp) of 3 samples showed similarity
with HBV with 90.8–96.3% of identity.
Conclusions. Serological and molecular data showed evidence of the circulation of a virus similar to hepatitis B virus in swine.