Serological and molecular evidence of hepadnavirus infection in swine

dc.creatorVieira, Yasmine R.
dc.creatorSilva, Marcelle F. M.
dc.creatorSantos, Débora R. L.
dc.creatorVieira, Antônio A.
dc.creatorZanella, Janice R. Ciacci
dc.creatorBarquero, Gonzalo
dc.creatorLago, Bárbara V. do
dc.creatorGomes, Selma A.
dc.creatorPinto, Marcelo A.
dc.creatorPaula, Vanessa S. de
dc.date2015-11-03T15:49:16Z
dc.date2015-11-03T15:49:16Z
dc.date2015
dc.date.accessioned2023-09-27T00:09:56Z
dc.date.available2023-09-27T00:09:56Z
dc.identifierVIEIRA, Yasmine R. et al. Serological and molecular evidence of hepadnavirus infection in swine. Annals of Agricultural and Environmental Medicine, v. 22, n.1, p.11–16, 2015.
dc.identifier1232-1966
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12110
dc.identifier10.5604/12321966.1141361
dc.identifier1898-2263
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898093
dc.descriptionAbstract Introduction and objective. Recently, investigations in a swine herd identified evidence of the existence of a novel member of the Hepadnavirus family endemic in swine. The aim of this study was to investigate the serological and molecular markers of Hepadnavirus circulation in Brazilian domestic swine and wild boar herds, and to evaluate the identity with HBV and other Hepadnaviruses reported previously. Materials and methods. For the study, 376 swine were screened for hepatitis B virus serological markers. Analyses were performed in serum samples using commercial enzyme-linked immunosorbent assay (ELISA) kits (DiaSorin®) for anti-HBc, HBsAg and anti-HBs. Reactive and undetermined swine serum samples were selected to perform DNA viral extraction (QIAamp DNA Mini Kit, Qiagen®), partial genome amplification and genome sequencing. Results. From 376 swine samples analysed, 28 (7.45%) were reactive to anti-HBc, 3 (0.80%) to HBsAg and 6 (1.6%) to anti-HBs. Besides, more 17 (4.52%) swine samples analyzed were classified in the grey zone of the EIA test to anti-HBc and 2 (0.53%) to HBsAg. From 49 samples molecularly analyzed after serological trial, 4 samples showed a positive result for the qualitative PCR for Hepadnavirus. Phylogenetic reconstruction using partial genome sequencing (360 bp) of 3 samples showed similarity with HBV with 90.8–96.3% of identity. Conclusions. Serological and molecular data showed evidence of the circulation of a virus similar to hepatitis B virus in swine.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNational Center for Biotechnology Information
dc.rightsopen access
dc.subjectDiagnostic
dc.subjectHepadnavirus
dc.subjectSwine
dc.subjectSuínos
dc.subjectDiagnóstico
dc.subjectVírus da Hepatite B
dc.titleSerological and molecular evidence of hepadnavirus infection in swine
dc.typeArticle


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