Article
Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System
Registro en:
HOLGUÍN, Angela Victoria et al. Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System. Viruses, v. 11, n. 2, p. 1-11, 2019.
1999-4915
10.3390/v11020188
Autor
Holguín, Angela Victoria
Cárdenas, Pablo
Peñaranda, Catalina Prada-
Leite, Laura Rabelo
Buitrago, Camila
Clavijo, Viviana
Oliveira, Guilherme Corrêa de
Leekitcharoenphon, Pimlapas
Aarestrup, Frank Møller
Vives, Martha J.
Resumen
Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and jSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy. 2022-01-01