dc.creator | Holguín, Angela Victoria | |
dc.creator | Cárdenas, Pablo | |
dc.creator | Peñaranda, Catalina Prada- | |
dc.creator | Leite, Laura Rabelo | |
dc.creator | Buitrago, Camila | |
dc.creator | Clavijo, Viviana | |
dc.creator | Oliveira, Guilherme Corrêa de | |
dc.creator | Leekitcharoenphon, Pimlapas | |
dc.creator | Aarestrup, Frank Møller | |
dc.creator | Vives, Martha J. | |
dc.date | 2019-11-06T14:01:23Z | |
dc.date | 2019-11-06T14:01:23Z | |
dc.date | 2019 | |
dc.date.accessioned | 2023-09-27T00:06:24Z | |
dc.date.available | 2023-09-27T00:06:24Z | |
dc.identifier | HOLGUÍN, Angela Victoria et al. Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System. Viruses, v. 11, n. 2, p. 1-11, 2019. | |
dc.identifier | 1999-4915 | |
dc.identifier | https://www.arca.fiocruz.br/handle/icict/36893 | |
dc.identifier | 10.3390/v11020188 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8897493 | |
dc.description | Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and jSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy. | |
dc.description | 2022-01-01 | |
dc.format | application/pdf | |
dc.language | eng | |
dc.publisher | MDPI | |
dc.rights | restricted access | |
dc.subject | Phage-therapy | |
dc.subject | Salmonella Enteritidis | |
dc.subject | Bacteria-phage coevolution | |
dc.subject | Antibiotics | |
dc.subject | Bacterial resistance | |
dc.title | Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System | |
dc.type | Article | |