dc.creatorHolguín, Angela Victoria
dc.creatorCárdenas, Pablo
dc.creatorPeñaranda, Catalina Prada-
dc.creatorLeite, Laura Rabelo
dc.creatorBuitrago, Camila
dc.creatorClavijo, Viviana
dc.creatorOliveira, Guilherme Corrêa de
dc.creatorLeekitcharoenphon, Pimlapas
dc.creatorAarestrup, Frank Møller
dc.creatorVives, Martha J.
dc.date2019-11-06T14:01:23Z
dc.date2019-11-06T14:01:23Z
dc.date2019
dc.date.accessioned2023-09-27T00:06:24Z
dc.date.available2023-09-27T00:06:24Z
dc.identifierHOLGUÍN, Angela Victoria et al. Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System. Viruses, v. 11, n. 2, p. 1-11, 2019.
dc.identifier1999-4915
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36893
dc.identifier10.3390/v11020188
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8897493
dc.descriptionBacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and jSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsrestricted access
dc.subjectPhage-therapy
dc.subjectSalmonella Enteritidis
dc.subjectBacteria-phage coevolution
dc.subjectAntibiotics
dc.subjectBacterial resistance
dc.titleHost Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System
dc.typeArticle


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