Article
Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
Registro en:
MARINS, Rachel Siqueira de Queiroz Simões; et al. Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy. International Research Journal of Biochemistry and Bioinformatics, v. 2, n.3, p.62-68, Mar. 2012.
2250-9941
Autor
Marins, Rachel Siqueira de Queiroz Simões
Cassiano, Keila Mara
Pereira, Sílvia Regina Ferreira Gonçalves
Nogueira, Denise Monnerat
Carvalho, Eulógio Queiroz de
Resumen
In the present study, we reported about papillomavirus (PV) clinical infection and viral latent
investigation in canines. Clinical and molecular analyses for detection of DNA PV infection, as well
as histopathology examination and cytogenetic evaluation were carried out in clinical lesions
canines. Blood sample from the both epithelial infection and the asymptomatic dogs had been
collected for molecular analysis. Amplification of DNA samples with the L1 gene from generic
primers were detected in skin biopsies and blood samples from canines healthy clinically or not. To
investigate the chromosomal fragility, we researched by cytogenetic technique in the peripheral
blood lymphocytes cultures and in the tumors cells cultures from canines presenting oral and
cutaneous warts lesions. The histological examinations of the skin biopsy specimens were
diagnosed like adenoma, carcinoma and papilloma. In some cases, besides the surgical removal,
autogenous vaccine derived from fresh warts of the same animal was indicated. The findings
documented as mucosal and cutaneous tropism, which the PV viral infection not confined only
epithelial sites, but also involved intracellular genome of host cell.