Article
PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil
Registro en:
SILVEIRA, A. P. S. da et al. PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 6, p. 843–847, dez. 2018.
1678-9849
10.1590/0037-8682-0040-2018
Autor
Silveira, Ana Paula Stefanello da
Vieira, Victor Bruno Duarte
Batalini, Leticia Surian
Carmo, Silvia Barbosa do
Friozi, Elisabete
Arruda, Eduardo José de
Lima Junior, Manoel Sebastião da Costa
Neitzke-Abreu, Herintha Coeto
Resumen
INTRODUCTION:
Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), and clinical signs were characterized.
METHODS:
PCR and parasitological tests were conducted.
RESULTS:
PCR was positive for Leishmania in 84.75%, and parasitological tests showed that 63.25% and 31.75% were positive for Leishmania and Ehrlichia, respectively. All animals showed more than three clinical signs. PCR results were negative for Leishmania in 15.25% of the samples.
CONCLUSIONS:
Conventional PCR of peripheral blood can be used for diagnosing canine visceral leishmaniasis in combination with other techniques, especially in uncertain cases that need species identification.