PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil

dc.creatorSilveira, Ana Paula Stefanello da
dc.creatorVieira, Victor Bruno Duarte
dc.creatorBatalini, Leticia Surian
dc.creatorCarmo, Silvia Barbosa do
dc.creatorFriozi, Elisabete
dc.creatorArruda, Eduardo José de
dc.creatorLima Junior, Manoel Sebastião da Costa
dc.creatorNeitzke-Abreu, Herintha Coeto
dc.date2019-03-28T13:09:43Z
dc.date2019-03-28T13:09:43Z
dc.date2018
dc.date.accessioned2023-09-26T23:42:11Z
dc.date.available2023-09-26T23:42:11Z
dc.identifierSILVEIRA, A. P. S. da et al. PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 6, p. 843–847, dez. 2018.
dc.identifier1678-9849
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32265
dc.identifier10.1590/0037-8682-0040-2018
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8893333
dc.descriptionINTRODUCTION: Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), and clinical signs were characterized. METHODS: PCR and parasitological tests were conducted. RESULTS: PCR was positive for Leishmania in 84.75%, and parasitological tests showed that 63.25% and 31.75% were positive for Leishmania and Ehrlichia, respectively. All animals showed more than three clinical signs. PCR results were negative for Leishmania in 15.25% of the samples. CONCLUSIONS: Conventional PCR of peripheral blood can be used for diagnosing canine visceral leishmaniasis in combination with other techniques, especially in uncertain cases that need species identification.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectCanine visceral leishmaniasis
dc.subjectPCR
dc.subjectSensitivity
dc.subjectPeripheral blood
dc.subjectAnimais
dc.subjectBrasil / epidemiologia
dc.subjectCoinfecção
dc.subjectDoenças do Cão / diagnóstico
dc.subjectDoenças do Cão / epidemiologia
dc.subjectCães
dc.subjectErliquiose / diagnóstico
dc.subjectErliquiose / epidemiologia
dc.subjectErliquiose / veterinária
dc.subjectDoenças endêmicas
dc.subjectFêmea
dc.subjectLeishmaniose Visceral / diagnóstico
dc.subjectLeishmaniose Visceral / epidemiologia
dc.subjectLeishmaniose Visceral / veterinária
dc.subjectMasculino
dc.subjectReação em Cadeia da Polimerase
dc.subjectSensibilidade e Especificidade
dc.titlePCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil
dc.typeArticle


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