Article
miR-6087 might regulate cell cycle–related mRNAs during cardiomyogenesis of hESCs
Registro en:
MACHADO, Hellen Cristine et al. miR-6087 might regulate cell cycle–related mRNAs during cardiomyogenesis of hESCs. Bioinformatics and Biology Insights, v. 17, p. 1-11, 2023.
1177-9322
Autor
Machado, Hellen Cristine
Bispo, Saloe
Dallagiovanna, Bruno
Resumen
MicroRNAs (miRNAs) are small noncoding RNAs that act as negative regulators of gene expression at the post-transcriptional level, promoting mRNA degradation or translation repression. Despite the well-described presence of miRNAs in various human tissues, there is still a lack of information about the relationship between miRNAs and the translation regulation in human embryonic stem cells (hESCs) during cardiomyogenesis. Here, we investigate RNA-seq data from hESCs, focusing on distinct stages of cardiomyogenesis and searching for polysome-bound miRNAs that could be involved in translational regulation. We identify miR-6087 as a differentially expressed miRNA at latest steps of cardiomyocyte differentiation. We analyzed the coexpression pattern between the differentially expressed mRNAs and miR-6087, evaluating whether they are predicted targets of the miRNA. We arranged the genes into an interaction network and identified BLM, RFC4, RFC3, and CCNA2 as key genes of the network. A post hoc analysis of the key genes suggests that miR-6087 could act as a regulator of the cell cycle in hESC during cardiomyogenesis.