Now showing items 1-10 of 157
TRAP-SEQ of eukaryotic translatomes applied to the detection of polysome-associated long noncoding RNAs
Translating ribosome affinity purification (TRAP) technology allows the isolation of polysomal complexes and the RNAs associated with at least one 80S ribosome. TRAP consists of the stabilization and affinity purification ...
Mammalian Staufen 1 is recruited to stress granules and impairs their assembly
(Company of Biologists, 2009-02)
Stress granules are cytoplasmic mRNA-silencing foci that form transiently during the stress response. Stress granules harbor abortive translation initiation complexes and are in dynamic equilibrium with translating polysomes. ...
Translation and Silencing in RNA Granules: a Tale of Sand Grains
The transcriptome at the synapse consists of thousands of messengers encoding several cellular functions, including a significant number of receptors and ion channels and associated proteins. The concerted translational ...
Polysome-seq as a Measure of Translational Profile from Deoxyhypusine Synthase Mutant in Saccharomyces cerevisiae
The profile of proteins observed in a cell is characterized by the control of gene expression, which has several regulation points acting individually or in concert, such as epigenetic, transcriptional, translational, ...
Stem Cells and the Translational Control of Differentiation: Following the Ribosome Footprints
(Susumu Minamisawa, 2016)
Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
(Nature Research, 2018)