Preprint
CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
Registro en:
RAPOSO, Jèssica Vasques et al. CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro. Research Square, p. 1 - 10, Oct. 2021.
Autor
Raposo, Jéssica Vasques
Barros, Luciana Rodrigues Carvalho
Torres, Lauana Ribas
Pinto, Rafaela Barbosa da Silva
Lopes, Amanda de Oliveira
Souza, Elen Mello de
Bonamino, Martin Hernan
Paula, Vanessa Salete de
Resumen
DOI: https://doi.org/10.21203/rs.3.rs-833312/v1 HSV-1 affects approximately 67% of the world population. Here, we sought to use the CRISPR / Cas9
system with the UL39 target, essential for virus replication. The sgRNA sequence was inserted into
plasmid (PX459-UL39). Vero cells were transfected with PX459-UL39, and inhibition of viral replication
was assessed 24 and 48 hours later using plaque assays and fluorescence and qPCR. Fluorescence
analyzes revealed the presence of anti-HSV-1 CRISPR/Cas9 within Vero cells, and qPCR showed that the
viral load decreased by> 95% of cells transfected with anti-HSV-1 CRISPR / Cas9. Our data demonstrate
the usefulness of the PX459-UL39 to inhibit HSV-1 infection.