CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro

dc.creatorRaposo, Jéssica Vasques
dc.creatorBarros, Luciana Rodrigues Carvalho
dc.creatorTorres, Lauana Ribas
dc.creatorPinto, Rafaela Barbosa da Silva
dc.creatorLopes, Amanda de Oliveira
dc.creatorSouza, Elen Mello de
dc.creatorBonamino, Martin Hernan
dc.creatorPaula, Vanessa Salete de
dc.date2023-05-10T13:14:18Z
dc.date2023-05-10T13:14:18Z
dc.date2021
dc.date.accessioned2023-09-26T23:19:27Z
dc.date.available2023-09-26T23:19:27Z
dc.identifierRAPOSO, Jèssica Vasques et al. CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro. Research Square, p. 1 - 10, Oct. 2021.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/58279
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8889313
dc.descriptionDOI: https://doi.org/10.21203/rs.3.rs-833312/v1
dc.descriptionHSV-1 affects approximately 67% of the world population. Here, we sought to use the CRISPR / Cas9 system with the UL39 target, essential for virus replication. The sgRNA sequence was inserted into plasmid (PX459-UL39). Vero cells were transfected with PX459-UL39, and inhibition of viral replication was assessed 24 and 48 hours later using plaque assays and fluorescence and qPCR. Fluorescence analyzes revealed the presence of anti-HSV-1 CRISPR/Cas9 within Vero cells, and qPCR showed that the viral load decreased by> 95% of cells transfected with anti-HSV-1 CRISPR / Cas9. Our data demonstrate the usefulness of the PX459-UL39 to inhibit HSV-1 infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherResearch Square
dc.rightsopen access
dc.subjectHuman alphaherpesvirus 1 (HSV-1)
dc.subjectCRISPR-associated (CRISPR/Cas)
dc.subjectHSV-1
dc.subjectsgRNA
dc.subjectFluorescence
dc.subjectCRISPR/Cas9
dc.titleCRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
dc.typePreprint


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