This study investigated the use of a virtual learning community (VLC-Bio) combined to an online teachers' professional development program. VLC-Bio enabled the sharing of biological knowledge, teaching methods and didactic resources. Although they presented a limited initial profile of internet use directed to socialization, the results indicated that participation in the VLC-Bio focused internet use for teaching and learning purposes. The VLC-Bio offered opportunities to develop the ability to learn from their peers about how to deal with matters of difficult approach in everyday school life, as well as of sharing resources for Biology education that are frequently lacking.