Article
RNA interference-mediated knockdown of CD49e (α5 integrin chain) in human thymic epithelial cells modulates the expression of multiple genes and decreases thymocyte adhesion
Registro en:
LACERDA, Leandra Linhares et al. RNA interference-mediated knockdown of CD49e (a5 integrin chain) in human thymic epithelial cells modulates the expression of multiple genes and decreases thymocyte adhesion. BMC Genomics, v.1, Suppl. 5, p. :S2, 13 p, 2010.
1471-2164
10.1186/1471-2164-11-S5-S2
Autor
Lacerda, Leandra Linhares
Alves, Marcelo Ribeiro
Nogueira, Ana Cristina Martins de Almeida
Cruz, Daniella Arêas Mendes da
Magalhães, Danielle Aparecida
Dardenne, Mireille
Passos, Geraldo Aleixo
Savino, Wilson
Resumen
From 5th International Conference of the Brazilian Association for Bioinformatics and Computational Biology
(X-meeting 2009)
Angra Dos Reis, RJ, Brazil. 18-22 October 2009 The thymus is a central lymphoid organ, in which bone marrow-derived T cell precursors undergo a complex process of maturation. Developing thymocytes interact with thymic microenvironment in a defined spatial order. A component of thymic microenvironment, the thymic epithelial cells, is crucial for the maturation of T-lymphocytes through cell-cell contact, cell matrix interactions and secretory of cytokines/chemokines. There is evidence that extracellular matrix molecules play a fundamental role in guiding differentiating thymocytes in both cortical and medullary regions of the thymic lobules. The interaction between the integrin α5β1 (CD49e/CD29; VLA-5) and fibronectin is relevant for thymocyte adhesion and migration within the thymic tissue. Our previous results have shown that adhesion of thymocytes to cultured TEC line is enhanced in the presence of fibronectin, and can be blocked with anti-VLA-5 antibody.