Article
Efficient in vitro expansion of JC virus-specific CD8+ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy
Registro en:
MARZOCCHETTI, Angela et al. Efficient in vitro expansion of JC virus-specific CD8+ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy. Virology, v. 383, n. 2, p. 173-177, Jan. 2009.
0042-6822
10.1016/j.virol.2008.10.046
Autor
Marzocchetti, Angela
Lima, Marco
Tompkins, Troy
Kavanagh, Daniel.G
Gandhi, Rajesh T.
O'Neill, David W.
Bhardwaj, Nina
Koralnik, Igor J.
Resumen
Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the brain caused by JC virus (JCV) for which there is no cure. PML patients who have JCV-specific CD8+ cytotoxic T lymphocytes (CTL) in their blood have a better clinical outcome. We compared JCV-specific CTL responses in vitro elicited either by JCV peptide-loaded dendritic cells (DC) or by direct peptide stimulation of lymphocytes from 20 HLA A*0201+ healthy controls, HIV+ and PML patients. JCV peptide-loaded DC elicited a stronger CTL expansion in 13/15 responders. DC can induce potent JCV-specific CTL response in vitro, and may constitute a promising approach for PML immunotherapy.