Article
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins
Registro en:
HENRIQUES, Bianca Santos et al. Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins. Front. Physiol., v. 11, Article 509310, 20 p, Jan. 2021.
1664-042X
10.3389/fphys.2020.509310
Autor
Henriques, Bianca Santos
Gomes, Bruno
Oliveira, Pedro Lagerblad
Garcia, Eloi de Souza
Azambuja, Patricia
Genta, Fernando Ariel
Resumen
Rhodnius prolixus is one important vector for the parasite Trypanosoma cruzi in Latin
America, where Chagas disease is a significant health issue. Although R. prolixus is
a model for investigations of vector–parasite interaction and transmission, not much
has been done recently to further comprehend its protein digestion. In this work, gut
proteolysis was characterized using new fluorogenic substrates, including optimum
pH, inhibition profiles, and tissue and temporal expression patterns. Each protease
possessed a particular tissue prevalence and activity cycle after feeding. Cathepsin L
had a higher activity in the posterior midgut lumen, being characterized by a plateau of
high activities during several days in the intermediate phase of digestion. Cathepsin D
showed high activity levels in the tissue homogenates and in the luminal content of the
posterior midgut, with a single peak 5 days after blood feeding. Aminopeptidases are
highly associated with the midgut wall, where the highest activity is located. Assays with
proteinaceous substrates as casein, hemoglobin, and serum albumin revealed different
activity profiles, with some evidence of biphasic temporal proteolytic patterns. Cathepsin
D genes are preferentially expressed in the anterior midgut, while cathepsin L genes are
mainly located in the posterior portion of the midgut, with specific sets of genes being
differently expressed in the initial, intermediate, or late phases of blood digestion.
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