Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins

dc.creatorHenriques, Bianca Santos
dc.creatorGomes, Bruno
dc.creatorOliveira, Pedro Lagerblad
dc.creatorGarcia, Eloi de Souza
dc.creatorAzambuja, Patricia
dc.creatorGenta, Fernando Ariel
dc.date2021-07-12T14:01:51Z
dc.date2021-07-12T14:01:51Z
dc.date2021
dc.date.accessioned2023-09-26T23:05:22Z
dc.date.available2023-09-26T23:05:22Z
dc.identifierHENRIQUES, Bianca Santos et al. Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins. Front. Physiol., v. 11, Article 509310, 20 p, Jan. 2021.
dc.identifier1664-042X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/48147
dc.identifier10.3389/fphys.2020.509310
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886709
dc.descriptionRhodnius prolixus is one important vector for the parasite Trypanosoma cruzi in Latin America, where Chagas disease is a significant health issue. Although R. prolixus is a model for investigations of vector–parasite interaction and transmission, not much has been done recently to further comprehend its protein digestion. In this work, gut proteolysis was characterized using new fluorogenic substrates, including optimum pH, inhibition profiles, and tissue and temporal expression patterns. Each protease possessed a particular tissue prevalence and activity cycle after feeding. Cathepsin L had a higher activity in the posterior midgut lumen, being characterized by a plateau of high activities during several days in the intermediate phase of digestion. Cathepsin D showed high activity levels in the tissue homogenates and in the luminal content of the posterior midgut, with a single peak 5 days after blood feeding. Aminopeptidases are highly associated with the midgut wall, where the highest activity is located. Assays with proteinaceous substrates as casein, hemoglobin, and serum albumin revealed different activity profiles, with some evidence of biphasic temporal proteolytic patterns. Cathepsin D genes are preferentially expressed in the anterior midgut, while cathepsin L genes are mainly located in the posterior portion of the midgut, with specific sets of genes being differently expressed in the initial, intermediate, or late phases of blood digestion.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectRhodnius prolixus
dc.subjectProtease
dc.subjectDigestão proteica
dc.subjectCathepsin L.
dc.subjectCathepsin D
dc.subjectRhodnius prolixus
dc.subjectProtease
dc.subjectProtein digestion
dc.subjectCathepsin L.
dc.subjectCathepsin D
dc.titleCharacterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins
dc.typeArticle


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