Article
Molecular typing of Campylobacter jejuni strains: comparison among four different techniques
Registro en:
FRAZÃO, Miliane Rodrigues et al. Molecular typing of Campylobacter jejuni strains: comparison among four different techniques. Brazilian Journal of Microbiology, v. 515, p. 519-525, Dec. 2020.
1517-8382
10.1007/s42770-019-00218-8
1678-4405
Autor
Frazão, Miliane Rodrigues
Souza, Roberto Antonio de
Medeiros, Marta Inês Cazentini
Duque, Sheila da Silva
Cao, Guojie
Alard, Marc William
Falcão, Juliana Pfrimer
Resumen
This study compared the ability of pulsed-field gel electrophoresis (PFGE), flaA small variable region (SVR) sequencing,
analysis of the clustered regularly interspaced short palindromic repeats locus by high resolution melting analysis (CRISPRHRMA),
and multilocus sequence typing (MLST) for typing 111 Campylobacter jejuni strains isolated from diverse sources
during 20 years in Brazil. For this, we used previous results obtained by PFGE and flaA-SVR sequencing from our research group
and performed CRISPR-HRMA and MLST typing for the first time. Furthermore, the discrimination index (DI) of each method
was accessed. The DI for PFGE, flaA-SVR sequencing, CRISPR-HRMA, and MLST was 0.980, 0.932, 0.868, and 0.931,
respectively. By PFGE and flaA-SVR sequencing, some strains from clinical and non-clinical sources and from humans and
animals presented ≥ 80% similarity. Similarly, some strains from different origins presented the same ST and CRISPR-HRMA
types. In conclusion, despite the different DI values, all assays provided the same epidemiological information suggesting that a
potential transmission may have occurred between C. jejuni from clinical and non-clinical sources and from animals and humans
in Brazil. Furthermore it was demonstrated the suitability of PFGE that should be used preferably together with MLST and/or
flaA-SVR sequencing for typing C. jejuni strains.