Molecular typing of Campylobacter jejuni strains: comparison among four different techniques

dc.creatorFrazão, Miliane Rodrigues
dc.creatorSouza, Roberto Antonio de
dc.creatorMedeiros, Marta Inês Cazentini
dc.creatorDuque, Sheila da Silva
dc.creatorCao, Guojie
dc.creatorAlard, Marc William
dc.creatorFalcão, Juliana Pfrimer
dc.date2021-01-15T18:04:16Z
dc.date2021-01-15T18:04:16Z
dc.date2020
dc.date.accessioned2023-09-26T23:03:23Z
dc.date.available2023-09-26T23:03:23Z
dc.identifierFRAZÃO, Miliane Rodrigues et al. Molecular typing of Campylobacter jejuni strains: comparison among four different techniques. Brazilian Journal of Microbiology, v. 515, p. 519-525, Dec. 2020.
dc.identifier1517-8382
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/45718
dc.identifier10.1007/s42770-019-00218-8
dc.identifier1678-4405
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886332
dc.descriptionThis study compared the ability of pulsed-field gel electrophoresis (PFGE), flaA small variable region (SVR) sequencing, analysis of the clustered regularly interspaced short palindromic repeats locus by high resolution melting analysis (CRISPRHRMA), and multilocus sequence typing (MLST) for typing 111 Campylobacter jejuni strains isolated from diverse sources during 20 years in Brazil. For this, we used previous results obtained by PFGE and flaA-SVR sequencing from our research group and performed CRISPR-HRMA and MLST typing for the first time. Furthermore, the discrimination index (DI) of each method was accessed. The DI for PFGE, flaA-SVR sequencing, CRISPR-HRMA, and MLST was 0.980, 0.932, 0.868, and 0.931, respectively. By PFGE and flaA-SVR sequencing, some strains from clinical and non-clinical sources and from humans and animals presented ≥ 80% similarity. Similarly, some strains from different origins presented the same ST and CRISPR-HRMA types. In conclusion, despite the different DI values, all assays provided the same epidemiological information suggesting that a potential transmission may have occurred between C. jejuni from clinical and non-clinical sources and from animals and humans in Brazil. Furthermore it was demonstrated the suitability of PFGE that should be used preferably together with MLST and/or flaA-SVR sequencing for typing C. jejuni strains.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedade Brasileira de Microbiologia
dc.rightsopen access
dc.subjectCampylobacter jejuni
dc.subjectCRISPR-HRMA
dc.subjectEletroforese de gel de campo pulsado
dc.subjectEpidemiologia molecular
dc.subjectCampylobacter jejuni
dc.subjectPulsed field gel electrophoresis
dc.subjectflaA-SVRsequencing
dc.subjectCRISPR-HRMA
dc.subjectMLST
dc.subjectMolecular epidemiology
dc.titleMolecular typing of Campylobacter jejuni strains: comparison among four different techniques
dc.typeArticle


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