Article
Evaluation of VITEK® 2 and MALDI-TOF/MS automated methodologies in the identification of atypical Listeria spp. isolated from food in different regions of Brazil
Registro en:
REIS, Christiane M. F. dos et al. the identification of atypical Listeria spp. isolated from food in different regions of Brazil. Journal of Microbiological Methods, v. 194, 106434, p. 1 - 6, Feb. 2022.
0167-7012
10.1016/j.mimet.2022.106434
Autor
Reis, Cristhiane M.F. dos
Ramos, Gustavo Luis de P.A.
Pereira, Rodrigo de Castro Lisbôa
Vallim, Deyse Christina
Costa, Leonardo Emanuel de Oliveira
Resumen
Listeria monocytogenes is a pathogen responsible for listeriosis, a foodborne disease with high mortality rates
(20–30%). It mainly affects the elderly, pregnant women, and immunocompromised people. Although not
pathogenic, the isolation and identification of Listeria innocua are critical since they can indicate L. monocytogenes’
presence as they are closely related and widely distributed in the environment and food processing
plants. The objective of this study was to evaluate the effectiveness of the automated methods VITEK® 2 and
MALDI-TOF/MS in identifying 94 strains of the genus Listeria with atypical identification profile. The resulting
identification by Polymerase Chain Reaction (PCR), using specific primers for the most common species of Listeria,
was considered the correct identification and presented a total of 31 strains identified as Listeria innocua
(LI), 54 as L. monocytogenes (LM), 8 as Listeria welshimeri (LW) and 1 as Listeria grayi (LG). The VITEK® 2
automated system correctly identified, on average, 79% of the LI strains, 16% of the LM strains, and 88.0% of the
LW strains. In the analysis by MALDI-TOF/MS, on average, 73% of LM strains were correctly identified, few LW
strains were correctly identified, and all LI strains were incorrectly identified. Both VITEK® 2 and MALDI-TOF/
MS correctly identified the LG strain in both analyzes. The results demonstrate that automated methodologies
could not discriminate atypical strains of the Listeria genus and point to the need for the use of complementary
tests, such as PCR and chromogenic media, for the correct identification of these strains. 2025-02
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