Article
Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation
Registro en:
FRANÇA, L. S. A. et al. Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation. Allergy Asthma and Immunology Research, v. 10, n. 4, p. 406-419, 2018.
2092-7355
10.4168/aair.2018.10.4.406
Autor
França, Luciana Souza de Aragão
Rocha, Viviane Costa Junqueira
Andrade, André Cronemberger
Costa, F H B
Vasconcelos, José Fernandes
Athanazio, Daniel Abensur
Silva, Daniela Nascimento
Santos, Emanuelle de Souza
Meira, Cássio Santana
Araújo, Cintia Figueiredo de
Cerqueira, Jéssica Vieira
Cardillo, Fabíola
Neves, Neuza Maria Alcântara
Soares, Milena Botelho Pereira
Pontes-de-Carvalho, Lain Carlos
Resumen
Programa de Excelência em Pesquisa (PROEP-CNPq). The use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. Methods: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte-
macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme-
linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs
were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of
BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. Results: Induction of TolDCs was efficiently achieved
as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production,
and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number
of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed
lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased
IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and
the lungs. Conclusions: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic
dendritic cells for the management of allergic conditions.