Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation

dc.creatorFrança, Luciana Souza de Aragão
dc.creatorRocha, Viviane Costa Junqueira
dc.creatorAndrade, André Cronemberger
dc.creatorCosta, F H B
dc.creatorVasconcelos, José Fernandes
dc.creatorAthanazio, Daniel Abensur
dc.creatorSilva, Daniela Nascimento
dc.creatorSantos, Emanuelle de Souza
dc.creatorMeira, Cássio Santana
dc.creatorAraújo, Cintia Figueiredo de
dc.creatorCerqueira, Jéssica Vieira
dc.creatorCardillo, Fabíola
dc.creatorNeves, Neuza Maria Alcântara
dc.creatorSoares, Milena Botelho Pereira
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.date2018-08-10T13:45:55Z
dc.date2018-08-10T13:45:55Z
dc.date2018
dc.date.accessioned2023-09-26T22:59:21Z
dc.date.available2023-09-26T22:59:21Z
dc.identifierFRANÇA, L. S. A. et al. Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation. Allergy Asthma and Immunology Research, v. 10, n. 4, p. 406-419, 2018.
dc.identifier2092-7355
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28055
dc.identifier10.4168/aair.2018.10.4.406
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8885568
dc.descriptionPrograma de Excelência em Pesquisa (PROEP-CNPq).
dc.descriptionThe use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. Methods: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte- macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme- linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. Results: Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. Conclusions: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions.
dc.formatapplication/pdf
dc.languageeng
dc.publisherThe Korean Academy of Asthma Allergy and Clinical Immunology
dc.rightsopen access
dc.subjectCélulas dendríticas
dc.subjectAsma
dc.subjectAlérgenos
dc.subjectÁcaros da poeira doméstica
dc.subjectImunoterapia
dc.subjectIndução de tolerância
dc.subjectDendritic cells
dc.subjectAsthma
dc.subjectAllergens
dc.subjectHouse dust mites
dc.subjectImmunotherapy
dc.subjectTolerance induction
dc.titleTolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation
dc.typeArticle


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