Article
HPLC-DAD-ESI-MS profile, antibacterial activity, and modulation of the activity of antibiotics by Carica papaya L. against Escherichia coli serotypes
Registro en:
GONDIM, Cícera Natalia Figueiredo Leite et al. HPLC-DAD-ESI-MS profile, antibacterial activity, and modulation of the activity of antibiotics by Carica papaya L. against Escherichia coli serotypes. Phytomedicine Plus, v. 2, n. 4, p. 1-6, 2022.
2667-0313
10.1016/j.phyplu.2022.100306
Autor
Gondim, Cícera Natalia Figueiredo Leite
Bezerra, Denise Aline Casimiro
Sampaio, Nadghia Figueiredo Leite
Alencar, Maria Anésia Sousa de
Costa, Roger Henrique Sousa da
Abreu, Lucas Silva
Silva, Joanda Paolla Raimundo e
Pereira Junior, Francisco Nascimento
Tavares, Josean Fechine
Silva, Marcelo Sobral da
Ribeiro Filho, Jaime
Coutinho, Henrique Douglas Melo
Resumen
Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico – FUNCAP. Background: Diarrhea is currently the second leading cause of death among children under five years old in the world and therefore, remains a global public health problem. Considering that most cases of diarrhea are due to gastrointestinal infections, and medicinal plants have been traditionally used to treat such conditions, this work aimed to evaluate the chemical composition, antibacterial activity, and antibiotic-enhancing properties of C. papaya against Escherichia coli, EPEC (Enteropathogenic E. coli), and ETEC (Enterotoxigenic E. coli) serotypes. Methods: The chemical profile of the aqueous extract obtained from C. papaya leaves was determined by HPLC-DAD-ESI-MS. The antibacterial and antibiotic-enhancing activities were evaluated through the determination of the MIC, following the microdilution method. Results: A total of 17 compounds were identified by HPLC-DAD-ESI-MS. However, the extract of C. papaya did not present an intrinsic antibacterial effect, in addition to showing no modulatory effect when associated with antibiotics. Conclusions: Despite the presence of bioactive compounds, the aqueous extract C. papaya does not present antibacterial or antibiotic-enhancing effects against the ETEC and EPEC strains of E. coli. Nevertheless, further research is required to determine if its isolated components are potential drug candidates for the therapy of infections caused by these strains.