Article
Immune response to LinB13, a lutzomyia intermedia salivary protein correlates with disease severity in tegumentary leishmaniasis
Registro en:
CARVALHO, Augusto M. et al. Immune response to LinB13, a lutzomyia intermedia salivary protein correlates with disease severity in tegumentary leishmaniasis. Clinical Infectious Diseases, p. 1-29, 2022.
1537-6591
10.1093/cid/ciac258
Autor
Carvalho, Augusto M.
Viana, Sayonara M.
Andrade, Bruno B.
Oliveira, Fabiano
Valenzuela, Jesus G.
Carvalho, Edgar M.
Oliveira, Camila I. de
Resumen
PROEP-IGM-Fiocruz Bahia. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Programa de Pesquisa Intramural do Instituto Nacional de Alergia e Doenças Infecciosas Background: We have previously shown that seropositivity to rLinB-13, a salivary protein from Lutzomyia intermedia, predicted sand fly exposure and was associated with increased risk of developing cutaneous leishmaniasis (CL). Methods: Herein, we investigated the cellular immune response to saliva from Lu. intermedia, using rLinB-13 as a surrogate antigen in naturally exposed individuals presenting positive serology to LinB-13. We also investigated the response to rLinB-13 in leishmaniasis patients, displaying active ulcers and positive PCR for L. braziliensis. Results: Peripheral blood mononuclear cells (PBMCs) stimulated in vitro with rLinB-13 secreted elevated levels of IL-10, IL-4, IL-1β, IL-1α, IL-6 and chemokines (CCL3, CCL4, CCL5 and CXCL5). CL, and disseminated leishmaniasis (DL) patients displayed a significantly higher IgG response to rLinB-13, compared to healthy subjects and anti-rLinB-13 IgG was positively correlated with the number of lesions in DL patients. Positive serology to rLinB-13 was also associated with chemotherapy failure. PBMCs from DL patients stimulated with rLINB-13 secreted significantly higher levels IL-10 and IL-1β compared to CL individuals. Conclusions: In this study, we observed an association between humoral and cellular immune response to the sand fly salivary protein rLinB-13 and disease severity in tegumentary leishmaniasis. This study brings evidence that immunity to rLinB-13 influences disease outcome in L. braziliensis infection and results indicate that positive serology to rLinB-13 IgG can be employed as marker of DL, an emerging and severe form of disease caused by L. braziliensis.