Immune response to LinB13, a lutzomyia intermedia salivary protein correlates with disease severity in tegumentary leishmaniasis

dc.creatorCarvalho, Augusto M.
dc.creatorViana, Sayonara M.
dc.creatorAndrade, Bruno B.
dc.creatorOliveira, Fabiano
dc.creatorValenzuela, Jesus G.
dc.creatorCarvalho, Edgar M.
dc.creatorOliveira, Camila I. de
dc.date2022-05-06T14:42:02Z
dc.date2022-05-06T14:42:02Z
dc.date2022
dc.date.accessioned2023-09-26T22:53:03Z
dc.date.available2023-09-26T22:53:03Z
dc.identifierCARVALHO, Augusto M. et al. Immune response to LinB13, a lutzomyia intermedia salivary protein correlates with disease severity in tegumentary leishmaniasis. Clinical Infectious Diseases, p. 1-29, 2022.
dc.identifier1537-6591
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/52557
dc.identifier10.1093/cid/ciac258
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8884344
dc.descriptionPROEP-IGM-Fiocruz Bahia. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Programa de Pesquisa Intramural do Instituto Nacional de Alergia e Doenças Infecciosas
dc.descriptionBackground: We have previously shown that seropositivity to rLinB-13, a salivary protein from Lutzomyia intermedia, predicted sand fly exposure and was associated with increased risk of developing cutaneous leishmaniasis (CL). Methods: Herein, we investigated the cellular immune response to saliva from Lu. intermedia, using rLinB-13 as a surrogate antigen in naturally exposed individuals presenting positive serology to LinB-13. We also investigated the response to rLinB-13 in leishmaniasis patients, displaying active ulcers and positive PCR for L. braziliensis. Results: Peripheral blood mononuclear cells (PBMCs) stimulated in vitro with rLinB-13 secreted elevated levels of IL-10, IL-4, IL-1β, IL-1α, IL-6 and chemokines (CCL3, CCL4, CCL5 and CXCL5). CL, and disseminated leishmaniasis (DL) patients displayed a significantly higher IgG response to rLinB-13, compared to healthy subjects and anti-rLinB-13 IgG was positively correlated with the number of lesions in DL patients. Positive serology to rLinB-13 was also associated with chemotherapy failure. PBMCs from DL patients stimulated with rLINB-13 secreted significantly higher levels IL-10 and IL-1β compared to CL individuals. Conclusions: In this study, we observed an association between humoral and cellular immune response to the sand fly salivary protein rLinB-13 and disease severity in tegumentary leishmaniasis. This study brings evidence that immunity to rLinB-13 influences disease outcome in L. braziliensis infection and results indicate that positive serology to rLinB-13 IgG can be employed as marker of DL, an emerging and severe form of disease caused by L. braziliensis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherOxford University Press
dc.rightsopen access
dc.subjectGravidade da doença
dc.subjectLeishmania braziliensis
dc.subjectLeishmaniose cutânea
dc.subjectLeishmaniose disseminada
dc.subjectSaliva do flebotomíneo
dc.subjectDisease severity
dc.subjectLeishmania braziliensis
dc.subjectCutaneous leishmaniasis
dc.subjectDisseminated leishmaniasis
dc.subjectSand fly saliva
dc.subjectLeishmania braziliensis
dc.subjectLeishmaniose cutânea
dc.subjectÍndice de Gravidade de Doença
dc.titleImmune response to LinB13, a lutzomyia intermedia salivary protein correlates with disease severity in tegumentary leishmaniasis
dc.typeArticle


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