Article
Comparative immunological recognition of proteins from New Guinea "C" dengue virus type 2 prototype and from a dengue virus type 2 strain isolated in the State of Rio de Janeiro, Brazil
Registro en:
CÔRTES, Luzia M. de C. et al. Comparative immunological recognition of proteins from New Guinea "C" dengue virus type 2 prototype and from a dengue virus type 2 strain isolated in the State of Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 3, p. 331-334, Apr. 2003.
0074-0276
10.1590/S0074-02762003000300007
1678-8060
Autor
Côrtes, Luzia M. de C.
Barth, Ortrud Monika
Pantoja, Josery R.
Alves, Carlos R.
Resumen
The protein profiles of the New Guinea “C” dengue virus type 2 (DENV-2)prototype and those of a Brazilian
DENV-2 isolated in the State of Rio de Janeiro in 1995 were compared. SDS-PAGE analysis showed that the virus
from Rio de Janeiro expresses NS5 (93.0 kDa), NS3 (66.8 kDa) E (62.4 kDa) and NS1 (41.2 kDa) proteins differently
from the New Guinea “C” virus. The immunoblot revealed specificity and antigenicity for the NS3 protein from
DENV-2 Rio de Janeiro mainly in primary infections, convalescent cases, and in secondary infections in both cases
and only antigenicity for E and NS1 proteins for both viruses in primary and secondary infections.