Article
Mycobacterium tuberculosis induces delayed lipid droplet accumulation in dendritic cells depending on bacterial viability and virulence
Registro en:
COSTA, Maria Fernanda de Souza et al. Mycobacterium tuberculosis induces delayed lipid droplet accumulation in dendritic cells depending on bacterial viability and virulence. Wiley,119, p.224-236, Dec. 2022.
1365-2958
10.1111/mmi.15023
Autor
Costa, Maria Fernanda de Souza
Dutra, Filipe Pereira
Deboosere, Nathalie
Jouny, Samuel
Ryul Song, Ok-
Iack, Guilherme
Souza, Andreia Lamoglia
Roma, Eric Henrique
Delorme, Vincent
Bozza, Patricia T.
Brodin, Priscille
Resumen
Tuberculosis remains a global health threat with high morbidity. Dendritic cells (DCs)
participate in the acute and chronic inflammatory responses to Mycobacterium tuberculosis (Mtb) by directing the adaptive immune response and are present in lung
granulomas. In macrophages, the interaction of lipid droplets (LDs) with mycobacteriacontaining phagosomes is central to host-pathogen interactions. However, the data
available for DCs are still a matter of debate. Here, we reported that bone marrowderived DCs (BMDCs) were susceptible to Mtb infection and replication at similar
rate to macrophages. Unlike macrophages, the analysis of gene expression showed
that Mtb infection induced a delayed increase in lipid droplet-related genes and proinflammatory response. Hence, LD accumulation has been observed by high-content
imaging in late periods. Infection of BMDCs with killed H37Rv demonstrated that
LD accumulation depends on Mtb viability. Moreover, infection with the attenuated
strains H37Ra and Mycobacterium bovis-BCG induced only an early transient increase
in LDs, whereas virulent Mtb also induced delayed LD accumulation. In addition, infection with the BCG strain with the reintroduced virulence RD1 locus induced higher LD
accumulation and bacterial replication when compared to parental BCG. Collectively,
our data suggest that delayed LD accumulation in DCs is dependent on mycobacterial
viability and virulence. 2025