Mycobacterium tuberculosis induces delayed lipid droplet accumulation in dendritic cells depending on bacterial viability and virulence

dc.creatorCosta, Maria Fernanda de Souza
dc.creatorDutra, Filipe Pereira
dc.creatorDeboosere, Nathalie
dc.creatorJouny, Samuel
dc.creatorRyul Song, Ok-
dc.creatorIack, Guilherme
dc.creatorSouza, Andreia Lamoglia
dc.creatorRoma, Eric Henrique
dc.creatorDelorme, Vincent
dc.creatorBozza, Patricia T.
dc.creatorBrodin, Priscille
dc.date2023-05-10T14:01:16Z
dc.date2023-05-10T14:01:16Z
dc.date2022
dc.date.accessioned2023-09-26T22:38:21Z
dc.date.available2023-09-26T22:38:21Z
dc.identifierCOSTA, Maria Fernanda de Souza et al. Mycobacterium tuberculosis induces delayed lipid droplet accumulation in dendritic cells depending on bacterial viability and virulence. Wiley,119, p.224-236, Dec. 2022.
dc.identifier1365-2958
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/58286
dc.identifier10.1111/mmi.15023
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881347
dc.descriptionTuberculosis remains a global health threat with high morbidity. Dendritic cells (DCs) participate in the acute and chronic inflammatory responses to Mycobacterium tuberculosis (Mtb) by directing the adaptive immune response and are present in lung granulomas. In macrophages, the interaction of lipid droplets (LDs) with mycobacteriacontaining phagosomes is central to host-pathogen interactions. However, the data available for DCs are still a matter of debate. Here, we reported that bone marrowderived DCs (BMDCs) were susceptible to Mtb infection and replication at similar rate to macrophages. Unlike macrophages, the analysis of gene expression showed that Mtb infection induced a delayed increase in lipid droplet-related genes and proinflammatory response. Hence, LD accumulation has been observed by high-content imaging in late periods. Infection of BMDCs with killed H37Rv demonstrated that LD accumulation depends on Mtb viability. Moreover, infection with the attenuated strains H37Ra and Mycobacterium bovis-BCG induced only an early transient increase in LDs, whereas virulent Mtb also induced delayed LD accumulation. In addition, infection with the BCG strain with the reintroduced virulence RD1 locus induced higher LD accumulation and bacterial replication when compared to parental BCG. Collectively, our data suggest that delayed LD accumulation in DCs is dependent on mycobacterial viability and virulence.
dc.description2025
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsopen access
dc.subjectMycobacterium tuberculosis
dc.subjectinduz gota lipídica retardada acúmulo
dc.subjectCélulas dendríticas
dc.subjectDependendo da viabilidade bacteriana e virulência
dc.subjectMycobacterium tuberculosis
dc.subjectInduces delayed lipid droplet accumulation
dc.subjectDendritic cells depending
dc.subjectBacterial viability and virulence
dc.titleMycobacterium tuberculosis induces delayed lipid droplet accumulation in dendritic cells depending on bacterial viability and virulence
dc.typeArticle


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